2BN6
P-Element Somatic Inhibitor Protein
Summary for 2BN6
| Entry DOI | 10.2210/pdb2bn6/pdb |
| Related | 2BN5 |
| Descriptor | PSI (1 entity in total) |
| Functional Keywords | splicing, protein-protein interaction, proline-rich peptide, nuclear protein |
| Biological source | DROSOPHILA MELANOGASTER (FRUIT FLY) |
| Total number of polymer chains | 1 |
| Total formula weight | 3796.14 |
| Authors | Ignjatovic, T.,Yang, J.C.,Butler, P.J.G.,Neuhaus, D.,Nagai, K. (deposition date: 2005-03-21, release date: 2005-07-06, Last modification date: 2024-05-15) |
| Primary citation | Ignjatovic, T.,Yang, J.C.,Butler, J.,Neuhaus, D.,Nagai, K. Structural Basis of the Interaction between P-Element Somatic Inhibitor and U1-70K Essential for the Alternative Splicing of P-Element Transposase. J.Mol.Biol., 351:52-, 2005 Cited by PubMed Abstract: P-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein. PubMed: 15990112DOI: 10.1016/J.JMB.2005.04.077 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
Download full validation report
