2BKD
Structure of the N-terminal domain of Fragile X Mental Retardation Protein
Summary for 2BKD
| Entry DOI | 10.2210/pdb2bkd/pdb |
| Related | 2FMR |
| Descriptor | Fragile X messenger ribonucleoprotein 1 (1 entity in total) |
| Functional Keywords | fmrp, protein-protein interaction, mrna transport, nuclear protein |
| Biological source | Homo sapiens (human) |
| Cellular location | Nucleus . Isoform 6: Cytoplasm . Isoform 9: Cytoplasm . Isoform 10: Nucleus . Isoform 11: Nucleus : Q06787 |
| Total number of polymer chains | 1 |
| Total formula weight | 15618.60 |
| Authors | Ramos, A.,Hollingworth, D.,Adinolfi, S.,Castets, M.,Kelly, G.,Frenkiel, T.A.,Bardoni, B.,Pastore, A. (deposition date: 2005-02-15, release date: 2006-01-18, Last modification date: 2024-11-06) |
| Primary citation | Ramos, A.,Hollingworth, D.,Adinolfi, S.,Castets, M.,Kelly, G.,Frenkiel, T.A.,Bardoni, B.,Pastore, A. The structure of the N-terminal domain of the fragile X mental retardation protein: a platform for protein-protein interaction. Structure, 14:21-31, 2006 Cited by PubMed Abstract: FMRP, whose lack of expression causes the X-linked fragile X syndrome, is a modular RNA binding protein thought to be involved in posttranslational regulation. We have solved the structure in solution of the N-terminal domain of FMRP (NDF), a functionally important region involved in multiple interactions. The structure consists of a composite fold comprising two repeats of a Tudor motif followed by a short alpha helix. The interactions between the three structural elements are essential for the stability of the NDF fold. Although structurally similar, the two repeats have different dynamic and functional properties. The second, more flexible repeat is responsible for interacting both with methylated lysine and with 82-FIP, one of the FMRP nuclear partners. NDF contains a 3D nucleolar localization signal, since destabilization of its fold leads to altered nucleolar localization of FMRP. We suggest that the NDF composite fold determines an allosteric mechanism that regulates the FMRP functions. PubMed: 16407062DOI: 10.1016/j.str.2005.09.018 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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