2BIF

6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE H256A MUTANT WITH F6P IN PHOSPHATASE ACTIVE SITE

2BIF の概要

• エントリーDOI: 10.2210/pdb2bif/pdb
• 分子名称: PROTEIN (6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE), octyl beta-D-glucopyranoside, 6-O-phosphono-beta-D-fructofuranose, ... (8 entities in total)
• 機能のキーワード: kinase, transferase (phospho), phosphatase, hydrolase (phospho), glycolysis, bifunctional enzyme, transferase, hydrolase
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 110452.00

構造登録者

Yuen, M.H.,Hasemann, C.A. (登録日: 1998-10-26, 公開日: 1999-02-16, 最終更新日: 2023-08-23)

• 主引用文献: Yuen, M.H.,Mizuguchi, H.,Lee, Y.H.,Cook, P.F.,Uyeda, K.,Hasemann, C.A.; Crystal structure of the H256A mutant of rat testis fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase. Fructose 6-phosphate in the active site leads to mechanisms for both mutant and wild type bisphosphatase activities.; J.Biol.Chem., 274:2176-2184, 1999; PubMed: 9890980; DOI: 10.1074/jbc.274.4.2176

実験手法

X-RAY DIFFRACTION (2.4 Å)