2BFV

MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI

2BFV の概要

• エントリーDOI: 10.2210/pdb2bfv/pdb
• 関連するPDBエントリー: 1BFV
• 分子名称: FV4155, ESTRIOL 3-(B-D-GLUCURONIDE), ... (4 entities in total)
• 機能のキーワード: immunoglobulin, fv fragment, steroid hormone, fine specificity
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26094.26

構造登録者

Trinh, C.H.,Phillips, S.E.V. (登録日: 1997-05-27, 公開日: 1997-12-03, 最終更新日: 2024-10-23)

主引用文献

Trinh, C.H.,Hemmington, S.D.,Verhoeyen, M.E.,Phillips, S.E.
Antibody fragment Fv4155 bound to two closely related steroid hormones: the structural basis of fine specificity.
Structure, 5:937-948, 1997

PubMed Abstract: The concentration of steroid glucuronides in serial samples of early morning urine (EMU) can be used to predict the fertile period in the female menstrual cycle. The monoclonal antibody 4155 has been used as a convenient means of measuring the concentration of steroid glucuronides in EMU, as it specifically recognises the steroid hormone estrone beta-D-glucuronide (E3G), with very high affinity, and the closely related hormone estriol 3-(beta-d-glucuronide) (EI3G), with reduced affinity. Although 4115 binds these hormones with different affinities, EI3G differs from E3G only in the addition of a hydroxyl group and reduction of an adjacent carbonyl. To investigate the structural basis of this fine binding specificity, we have determined the crystal structures of the variable fragment (Fv) of 4155 in complex with each of these hormones.

PubMed: 9261086
DOI: 10.1016/S0969-2126(97)00247-5

実験手法

X-RAY DIFFRACTION (2.5 Å)