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1BFV

MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI

Summary for 1BFV
Entry DOI10.2210/pdb1bfv/pdb
DescriptorFV4155, ZINC ION, ESTRIOL 3-(B-D-GLUCURONIDE), ... (5 entities in total)
Functional Keywordsimmunoglobulin, fv fragment, steroid hormone, fine specificity
Biological sourceMus musculus (house mouse)
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Total number of polymer chains2
Total formula weight26290.49
Authors
Trinh, C.H.,Phillips, S.E.V. (deposition date: 1997-05-27, release date: 1997-12-03, Last modification date: 2024-10-23)
Primary citationTrinh, C.H.,Hemmington, S.D.,Verhoeyen, M.E.,Phillips, S.E.
Antibody fragment Fv4155 bound to two closely related steroid hormones: the structural basis of fine specificity.
Structure, 5:937-948, 1997
Cited by
PubMed Abstract: The concentration of steroid glucuronides in serial samples of early morning urine (EMU) can be used to predict the fertile period in the female menstrual cycle. The monoclonal antibody 4155 has been used as a convenient means of measuring the concentration of steroid glucuronides in EMU, as it specifically recognises the steroid hormone estrone beta-D-glucuronide (E3G), with very high affinity, and the closely related hormone estriol 3-(beta-d-glucuronide) (EI3G), with reduced affinity. Although 4115 binds these hormones with different affinities, EI3G differs from E3G only in the addition of a hydroxyl group and reduction of an adjacent carbonyl. To investigate the structural basis of this fine binding specificity, we have determined the crystal structures of the variable fragment (Fv) of 4155 in complex with each of these hormones.
PubMed: 9261086
DOI: 10.1016/S0969-2126(97)00247-5
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.1 Å)
Structure validation

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