2BFV
MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04 |
| Detector | RIGAKU |
| Spacegroup name | P 32 |
| Unit cell lengths | 53.600, 53.600, 83.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.176 * |
| Rwork | 0.187 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nbv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.037 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((ROTAVATA)) |
| Phasing software | AMoRE |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.064 * | 0.225 * |
| Total number of observations | 12233 * | |
| Number of reflections | 7836 | |
| <I/σ(I)> | 7.21 | 2.6 |
| Completeness [%] | 86.9 | 87.5 |
| Redundancy | 1.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | PROTEIN WAS CRYSTALLIZED FROM 15% (W/V) PEG 4000, 100MM NA ACETATE AND 50MM TRIS HCL PH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5-3 (mg/ml) | |
| 2 | 1 | drop | Na acetate | 0.05 (M) | |
| 3 | 1 | drop | Tris-HCl | 0.025 (M) | |
| 4 | 1 | drop | PEG4000 | 7.5 (%(w/v)) | |
| 5 | 1 | reservoir | Na acetate | 0.1 (M) | |
| 6 | 1 | reservoir | Tris-HCl | 0.05 (M) | |
| 7 | 1 | reservoir | PEG4000 | 15 (%(w/v)) |
