2BEX

Crystal structure of Placental Ribonuclease Inhibitor in complex with Human Eosinophil Derived Neurotoxin at 2A resolution

2BEX の概要

• エントリーDOI: 10.2210/pdb2bex/pdb
• 関連するPDBエントリー: 1A4Y 1GQV 1HI2 1HI3 1HI4 1HI5 1K2A
• 分子名称: RIBONUCLEASE INHIBITOR, NONSECRETORY RIBONUCLEASE, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: hydrolase/inhibitor, complex (inhibitor-nuclease), ribonuclease inhibitor, eosinophil derived neurotoxin, rnase 2, luecine-rich repeats, protein-protein interaction, molecular recognition, hydrolase-inhibitor complex
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Cytoplasm: P13489; Lysosome (Probable): P10153
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 131207.95

構造登録者

Iyer, S.,Holloway, D.E.,Kumar, K.,Shapiro, R.,Acharya, K.R. (登録日: 2004-12-01, 公開日: 2005-03-17, 最終更新日: 2024-11-13)

主引用文献

Iyer, S.,Holloway, D.E.,Kumar, K.,Shapiro, R.,Acharya, K.R.
Molecular Recognition of Human Eosinophil-Derived Neurotoxin (Rnase 2) by Placental Ribonuclease Inhibitor
J.Mol.Biol., 347:637-, 2005

PubMed Abstract: Placental ribonuclease inhibitor (RI) binds diverse mammalian RNases with dissociation constants that are in the femtomolar range. Previous studies on the complexes of RI with RNase A and angiogenin revealed that RI utilises largely distinctive interactions to achieve high affinity for these two ligands. Here we report a 2.0 angstroms resolution crystal structure of RI in complex with a third ligand, eosinophil-derived neurotoxin (EDN), and a mutational analysis based on this structure. The RI-EDN interface is more extensive than those of the other two complexes and contains a considerably larger set of interactions. Few of the contacts present in the RI-angiogenin complex are replicated; the correspondence to the RI-RNase A complex is somewhat greater, but still modest. The energetic contributions of various interface regions differ strikingly from those in the earlier complexes. These findings provide insight into the structural basis for the unusual combination of high avidity and relaxed stringency that RI displays.

PubMed: 15755456
DOI: 10.1016/J.JMB.2005.01.035

実験手法

X-RAY DIFFRACTION (1.99 Å)