2BCK

Crystal Structure of HLA-A*2402 Complexed with a telomerase peptide

2BCK の概要

• エントリーDOI: 10.2210/pdb2bck/pdb
• 分子名称: HLA class I histocompatibility antigen, A-24 alpha chain, Beta-2-microglobulin, Telomerase reverse transcriptase, ... (6 entities in total)
• 機能のキーワード: immunoglobulin domains, beta barrels, mhc fold, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P05534; Secreted: P61769; Nucleus, nucleolus: O14746
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 94392.49

構造登録者

Rizkallah, P.J.,Jakobsen, B.K.,Cole, D.K.,Gao, G.F. (登録日: 2005-10-19, 公開日: 2006-01-10, 最終更新日: 2024-10-23)

主引用文献

Cole, D.K.,Rizkallah, P.J.,Gao, F.,Watson, N.I.,Boulter, J.M.,Bell, J.I.,Sami, M.,Gao, G.F.,Jakobsen, B.K.
Crystal structure of HLA-A*2402 complexed with a telomerase peptide.
Eur.J.Immunol., 36:170-179, 2006

PubMed Abstract: HLA-A*2402 is the most commonly expressed HLA allele in oriental populations. It is also widely expressed in the Caucasian population, making it one of, if not the most abundant HLA I types. In order to study its structure in terms of overall fold and peptide presentation, a soluble form of this HLA I (alpha1, alpha2, alpha3 and beta(2)m domains) has been expressed, refolded and crystallized in complex with a cancer-related telomerase peptide (VYGFVRACL), and its structure has been solved to 2.8 A resolution. The overall structure of HLA-A*2402 is virtually identical to other reported peptide-HLA I structures. However, there are distinct features observable from this structure at the HLA I peptide binding pockets. The size and depth of pocket B makes it highly suitable for binding to large aromatic side chains, which explains the high prevalence of tyrosine at peptide position 2. Also, for HLA binding at peptide position 5, there is an additional anchor point, which allows the proximal amino acids to protrude out, providing a prominent feature for TCR interaction. Finally, pocket F allows the anchor residue at position 9 to be bound unusually deeply within the HLA structure.

PubMed: 16323248
DOI: 10.1002/eji.200535424

実験手法

X-RAY DIFFRACTION (2.8 Å)