2BCJ

Crystal Structure of G Protein-Coupled Receptor Kinase 2 in Complex with Galpha-q and Gbetagamma Subunits

2BCJ の概要

• エントリーDOI: 10.2210/pdb2bcj/pdb
• 関連するPDBエントリー: 1OMW 1YM7
• 分子名称: G-protein-coupled receptor kinase 2, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, ... (8 entities in total)
• 機能のキーワード: peripheral membrane complex, protein kinase, rgs domain, wd40 protein, heterotrimeric g protein, transferase-hydrolase complex, transferase/hydrolase
• 由来する生物種: Bos taurus (Bovine); 詳細
• 細胞内の位置: Cytoplasm : P21146; Cell membrane ; Lipid-anchor ; Cytoplasmic side : P63212; Nucleus : P21279
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 166776.15

構造登録者

Tesmer, J.J.G. (登録日: 2005-10-19, 公開日: 2005-12-20, 最終更新日: 2024-10-16)

主引用文献

Tesmer, V.M.,Kawano, T.,Shankaranarayanan, A.,Kozasa, T.,Tesmer, J.J.G.
Snapshot of Activated G Proteins at the Membrane: The Gq-GRK2-G Complex
Science, 310:1686-1690, 2005

PubMed Abstract: G protein-coupled receptor kinase 2 (GRK2) plays a key role in the desensitization of G protein-coupled receptor signaling by phosphorylating activated heptahelical receptors and by sequestering heterotrimeric G proteins. We report the atomic structure of GRK2 in complex with Galphaq and Gbetagamma, in which the activated Galpha subunit of Gq is fully dissociated from Gbetagamma and dramatically reoriented from its position in the inactive Galphabetagamma heterotrimer. Galphaq forms an effector-like interaction with the GRK2 regulator of G protein signaling (RGS) homology domain that is distinct from and does not overlap with that used to bind RGS proteins such as RGS4.

PubMed: 16339447
DOI: 10.1126/science.1118890

実験手法

X-RAY DIFFRACTION (3.061 Å)