2B6G

RNA recognition by the Vts1 SAM domain

Summary for 2B6G

• Entry DOI: 10.2210/pdb2b6g/pdb
• Related: 2D3D
• NMR Information: BMRB: 6956
• Descriptor: 5'-R(*GP*GP*AP*GP*GP*CP*UP*CP*UP*GP*GP*CP*AP*GP*CP*UP*UP*UP*C)-3', Vts1p (2 entities in total)
• Functional Keywords: alpha-helix, pentaloop, hairpin, rna binding protein
• Biological source: Saccharomyces cerevisiae (baker's yeast); More
• Total number of polymer chains: 2
• Total formula weight: 19183.49

Authors

Donaldson, L.W.,Johnson, P.E. (deposition date: 2005-10-01, release date: 2006-01-24, Last modification date: 2024-05-22)

Primary citation

Johnson, P.E.,Donaldson, L.W.
RNA recognition by the Vts1p SAM domain
Nat.Struct.Mol.Biol., 13:177-178, 2006

PubMed Abstract: The putative yeast post-transcriptional regulator Vts1p and its related protein Smaug, from Drosophila melanogaster, each use a sterile alpha motif (SAM) domain to bind an RNA hairpin termed the Smaug recognition element (SRE). Here, we present the NMR structures of the Vts1p-SRE complex and the free SRE. Structural highlights include the direct recognition of a guanine base and the formation or stabilization of a base pair in the SRE loop.

PubMed: 16429155
DOI: 10.1038/nsmb1039

Experimental method

SOLUTION NMR