2B5L

Crystal Structure of DDB1 In Complex with Simian Virus 5 V Protein

2B5L の概要

• エントリーDOI: 10.2210/pdb2b5l/pdb
• 関連するPDBエントリー: 2B5M 2B5N
• 分子名称: damage-specific DNA binding protein 1, Nonstructural protein V, ZINC ION (3 entities in total)
• 機能のキーワード: ddb1, sv5-v, beta propeller, propeller cluster, zinc finger, protein binding-viral protein complex, protein binding/viral protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 302426.71

構造登録者

Li, T.,Chen, X.,Garbutt, K.C.,Zhou, P.,Zheng, N. (登録日: 2005-09-28, 公開日: 2006-02-28, 最終更新日: 2024-11-13)

主引用文献

Li, T.,Chen, X.,Garbutt, K.C.,Zhou, P.,Zheng, N.
Structure of DDB1 in complex with a paramyxovirus V protein: viral hijack of a propeller cluster in ubiquitin ligase.
Cell(Cambridge,Mass.), 124:105-117, 2006

PubMed Abstract: The DDB1-Cul4A ubiquitin ligase complex promotes protein ubiquitination in diverse cellular functions and is reprogrammed by the V proteins of paramyxoviruses to degrade STATs and block interferon signaling. Here we report the crystal structures of DDB1 alone and in complex with the simian virus 5 V protein. The DDB1 structure reveals an intertwined three-propeller cluster, which contains two tightly coupled beta propellers with a large pocket in between and a third beta propeller flexibly attached on the side. The rigid double-propeller fold of DDB1 is targeted by the viral V protein, which inserts an entire helix into the double-propeller pocket, whereas the third propeller domain docks DDB1 to the N terminus of the Cul4A scaffold. Together, these results not only provide structural insights into how the virus hijacks the DDB1-Cul4A ubiquitin ligase but also establish a structural framework for understanding the multiple functions of DDB1 in the uniquely assembled cullin-RING E3 machinery.

PubMed: 16413485
DOI: 10.1016/j.cell.2005.10.033

実験手法

X-RAY DIFFRACTION (2.85 Å)