2B2Y

Tandem chromodomains of human CHD1

2B2Y の概要

• エントリーDOI: 10.2210/pdb2b2y/pdb
• 関連するPDBエントリー: 1kna 1kne 1pdq 1q3l 2b2t 2b2u 2b2v 2b2w
• 分子名称: Chromodomain-helicase-DNA-binding protein 1 (3 entities in total)
• 機能のキーワード: chd, chromodomain, three stranded antiparallel beta sheet, alpha helix linker, peptide binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : O14646 O14646
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 57987.82

構造登録者

Flanagan IV, J.F.,Mi, L.-Z.,Chruszcz, M.,Cymborowski, M.,Clines, K.L.,Kim, Y.,Minor, W.,Rastinejad, F.,Khorasanizadeh, S. (登録日: 2005-09-19, 公開日: 2005-12-27, 最終更新日: 2023-08-23)

主引用文献

Flanagan, J.F.,Mi, L.Z.,Chruszcz, M.,Cymborowski, M.,Clines, K.L.,Kim, Y.,Minor, W.,Rastinejad, F.,Khorasanizadeh, S.
Double chromodomains cooperate to recognize the methylated histone H3 tail.
Nature, 438:1181-1185, 2005

PubMed Abstract: Chromodomains are modules implicated in the recognition of lysine-methylated histone tails and nucleic acids. CHD (for chromo-ATPase/helicase-DNA-binding) proteins regulate ATP-dependent nucleosome assembly and mobilization through their conserved double chromodomains and SWI2/SNF2 helicase/ATPase domain. The Drosophila CHD1 localizes to the interbands and puffs of the polytene chromosomes, which are classic sites of transcriptional activity. Other CHD isoforms (CHD3/4 or Mi-2) are important for nucleosome remodelling in histone deacetylase complexes. Deletion of chromodomains impairs nucleosome binding and remodelling by CHD proteins. Here we describe the structure of the tandem arrangement of the human CHD1 chromodomains, and its interactions with histone tails. Unlike HP1 and Polycomb proteins that use single chromodomains to bind to their respective methylated histone H3 tails, the two chromodomains of CHD1 cooperate to interact with one methylated H3 tail. We show that the human CHD1 double chromodomains target the lysine 4-methylated histone H3 tail (H3K4me), a hallmark of active chromatin. Methylammonium recognition involves two aromatic residues, not the three-residue aromatic cage used by chromodomains of HP1 and Polycomb proteins. Furthermore, unique inserts within chromodomain 1 of CHD1 block the expected site of H3 tail binding seen in HP1 and Polycomb, instead directing H3 binding to a groove at the inter-chromodomain junction.

PubMed: 16372014
DOI: 10.1038/nature04290

実験手法

X-RAY DIFFRACTION (2.35 Å)