2B2Y
Tandem chromodomains of human CHD1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-15 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.270, 55.031, 101.550 |
Unit cell angles | 90.00, 112.42, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.350 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2B2T without chain D |
RMSD bond length | 0.007 |
RMSD bond angle | 1.370 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.300 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 21913 | |
<I/σ(I)> | 11.6 | 3.2 |
Completeness [%] | 91.1 | 76.3 |
Redundancy | 3.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 283 | 4% PEG3350, 0.05M HEPES, 10mM BTP, pH 8.0, 12.5mM NaCl, 5mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 283K, pH 8.00 |