2AZ1

Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum

2AZ1 の概要

• エントリーDOI: 10.2210/pdb2az1/pdb
• 関連するPDBエントリー: 1NUE 2AZ3
• 分子名称: Nucleoside diphosphate kinase, CALCIUM ION (3 entities in total)
• 機能のキーワード: halophilic, surface charges, transferase
• 由来する生物種: Halobacterium salinarum
• 細胞内の位置: Cytoplasm : P61136
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 121749.34

構造登録者

Besir, H.,Zeth, K.,Bracher, A.,Heider, U.,Ishibashi, M.,Tokunaga, M.,Oesterhelt, D. (登録日: 2005-09-09, 公開日: 2005-12-20, 最終更新日: 2023-10-25)

主引用文献

Besir, H.,Zeth, K.,Bracher, A.,Heider, U.,Ishibashi, M.,Tokunaga, M.,Oesterhelt, D.
Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum
Febs Lett., 579:6595-6600, 2005

PubMed Abstract: Nucleoside diphosphate kinase from the halophilic archaeon Halobacterium salinarum was crystallized in a free state and a substrate-bound form with CDP. The structures were solved to a resolution of 2.35 and 2.2A, respectively. Crystals with the apo-form were obtained with His6-tagged enzyme, whereas the untagged form was used for co-crystallization with the nucleotide. Crosslinking under different salt and pH conditions revealed a stronger oligomerization tendency for the tagged protein at low and high salt concentrations. The influence of the His6-tag on the halophilic nature of the enzyme is discussed on the basis of the observed structural properties.

PubMed: 16293253
DOI: 10.1016/j.febslet.2005.10.052

実験手法

X-RAY DIFFRACTION (2.35 Å)