2ALC
ETHANOL REGULON TRANSCRIPTIONAL ACTIVATOR DNA-BINDING DOMAIN FROM ASPERGILLUS NIDULANS
Summary for 2ALC
| Entry DOI | 10.2210/pdb2alc/pdb |
| Descriptor | PROTEIN (ETHANOL REGULON TRANSCRIPTIONAL ACTIVATOR), ZINC ION (2 entities in total) |
| Functional Keywords | zinc binuclear cluster, dna-binding, transcriptional activator, dna binding protein |
| Biological source | Emericella nidulans |
| Cellular location | Nucleus: P21228 |
| Total number of polymer chains | 1 |
| Total formula weight | 7711.17 |
| Authors | Cerdan, R.,Cahuzac, B.,Felenbok, B.,Guittet, E. (deposition date: 1999-01-20, release date: 2000-01-21, Last modification date: 2023-12-27) |
| Primary citation | Cerdan, R.,Cahuzac, B.,Felenbok, B.,Guittet, E. NMR solution structure of AlcR (1-60) provides insight in the unusual DNA binding properties of this zinc binuclear cluster protein. J.Mol.Biol., 295:729-736, 2000 Cited by PubMed Abstract: The three-dimensional structure of the DNA-binding domain (residues 1-60) of the ethanol regulon transcription factor AlcR from Aspergillus nidulans has been solved by NMR. This domain belongs to the zinc binuclear cluster class. Although the core of the protein is similar to previously characterized structures, consisting of two helices organized around a Zn(2)Cys(6 )motif, the present structure presents important variations, among them the presence of two supplementary helices. This structure gives new insight into the understanding of the AlcR specificities in DNA binding such as longer consensus half-sites, in vitro monomeric binding but in vivo multiple repeat transcriptional activation, either in direct or inverse orientations. The presence of additional contacts of the protein with its DNA target can be predicted from a model proposed for the interaction with the consensus DNA target. The clustering of accessible negative charges on helix 2 delineates a possible interaction site for other determinants of the transcriptional machinery, responsible for the fine tuning of the selection of the AlcR cognate sites. PubMed: 10656785DOI: 10.1006/jmbi.1999.3417 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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