2AHE

Crystal structure of a soluble form of CLIC4. intercellular chloride ion channel

2AHE の概要

• エントリーDOI: 10.2210/pdb2ahe/pdb
• 関連するPDBエントリー: 1K0M
• 分子名称: Chloride intracellular channel protein 4 (2 entities in total)
• 機能のキーワード: glutathione-s-transferase superfamily, clic4, ncc27, chloride ion channel, metal transport
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, cytoskeleton, centrosome: Q9Y696
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30352.81

構造登録者

Littler, D.R.,Assaad, N.N.,Harrop, S.J.,Brown, L.J.,Pankhurst, G.J.,Luciani, P.,Aguilar, M.-I.,Mazzanti, M.,Berryman, M.A.,Breit, S.N.,Curmi, P.M.G. (登録日: 2005-07-28, 公開日: 2005-08-16, 最終更新日: 2023-10-25)

主引用文献

Littler, D.R.,Assaad, N.N.,Harrop, S.J.,Brown, L.J.,Pankhurst, G.J.,Luciani, P.,Aguilar, M.-I.,Mazzanti, M.,Berryman, M.A.,Breit, S.N.,Curmi, P.M.G.
Crystal structure of the soluble form of the redox-regulated chloride ion channel protein CLIC4.
FEBS J., 272:4996-5007, 2005

PubMed Abstract: The structure of CLIC4, a member of the CLIC family of putative intracellular chloride ion channel proteins, has been determined at 1.8 Angstroms resolution by X-ray crystallography. The protein is monomeric and it is structurally similar to CLIC1, belonging to the GST fold class. Differences between the structures of CLIC1 and CLIC4 are localized to helix 2 in the glutaredoxin-like N-terminal domain, which has previously been shown to undergo a dramatic structural change in CLIC1 upon oxidation. The structural differences in this region correlate with the sequence differences, where the CLIC1 sequence appears to be atypical of the family. Purified, recombinant, wild-type CLIC4 is shown to bind to artificial lipid bilayers, induce a chloride efflux current when associated with artificial liposomes and produce an ion channel in artificial bilayers with a conductance of 30 pS. Membrane binding is enhanced by oxidation of CLIC4 while no channels were observed via tip-dip electrophysiology in the presence of a reducing agent. Thus, recombinant CLIC4 appears to be able to form a redox-regulated ion channel in the absence of any partner proteins.

PubMed: 16176272
DOI: 10.1111/j.1742-4658.2005.04909.x

実験手法

X-RAY DIFFRACTION (1.8 Å)