2ADB

Solution structure of Polypyrimidine Tract Binding protein RBD2 complexed with CUCUCU RNA

Summary for 2ADB

• Entry DOI: 10.2210/pdb2adb/pdb
• Related: 2AD9 2ADC
• Descriptor: 5'-R(*CP*UP*CP*UP*CP*U)-3', Polypyrimidine tract-binding protein 1 (2 entities in total)
• Functional Keywords: rbd, rrm, protein-rna complex, rna binding protein-rna complex, rna binding protein/rna
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus: P26599
• Total number of polymer chains: 2
• Total formula weight: 18167.62

Authors

Oberstrass, F.C.,Auweter, S.D.,Erat, M.,Hargous, Y.,Henning, A.,Wenter, P.,Reymond, L.,Pitsch, S.,Black, D.L.,Allain, F.H.T. (deposition date: 2005-07-20, release date: 2005-10-04, Last modification date: 2024-05-29)

Primary citation

Oberstrass, F.C.,Auweter, S.D.,Erat, M.,Hargous, Y.,Henning, A.,Wenter, P.,Reymond, L.,Amir-Ahmady, B.,Pitsch, S.,Black, D.L.,Allain, F.H.T.
Structure of PTB bound to RNA: specific binding and implications for splicing regulation
Science, 309:2054-2057, 2005

PubMed Abstract: The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA binding protein involved in multiple aspects of messenger RNA metabolism, including the repression of alternative exons. We have determined the solution structures of the four RNA binding domains (RBDs) of PTB, each bound to a CUCUCU oligonucleotide. Each RBD binds RNA with a different binding specificity. RBD3 and RBD4 interact, resulting in an antiparallel orientation of their bound RNAs. Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor.

PubMed: 16179478
DOI: 10.1126/science.1114066

Experimental method

SOLUTION NMR