2AD6

crystal structure of methanol dehydrogenase from M. W3A1 (form C)

2AD6 の概要

• エントリーDOI: 10.2210/pdb2ad6/pdb
• 関連するPDBエントリー: 1G72 2AD7 2AD8 4AAH
• 分子名称: Methanol dehydrogenase subunit 1, Methanol dehydrogenase subunit 2, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: methanol dehydrogenase, pqq configuration, native, oxidoreductase
• 由来する生物種: Methylophilus methylotrophus; 詳細
• 細胞内の位置: Cell inner membrane; Peripheral membrane protein; Periplasmic side: P38539 P38540
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 141282.96

構造登録者

Li, J.,Gan, J.-H.,Xia, Z.-X.,Mathews, F.S. (登録日: 2005-07-20, 公開日: 2006-07-25, 最終更新日: 2024-11-13)

主引用文献

Li, J.,Gan, J.-H.,Mathews, F.S.,Xia, Z.-X.
The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase
Biochem.Biophys.Res.Commun., 406:621-626, 2011

PubMed Abstract: Methanol dehydrogenase is a heterotetrameric enzyme containing the prosthetic group pyrroloquinoline quinone (PQQ), which catalyzes the oxidation of methanol to formaldehyde. The crystal structure of methanol dehydrogenase from Methylophilus W3A1, previously determined at high resolution, exhibits a non-planar configuration of the PQQ ring system and lends support for a hydride transfer mechanism of the enzymatic reaction catalyzed by the enzyme. To investigate why PQQ is in the C5-reduced form and to better understand the catalytic mechanism of the enzyme, three structures of this enzyme in a new crystal form have been determined at higher resolution. Two of the three crystals were grown in the presence of 1 and 50 mM methanol, respectively, both structures of which show non-planar configurations of the PQQ ring system, confirming the previous conclusion; the other was crystallized in the presence of 50 mM ethanol, the structure of which displays a planar ring system for PQQ. Comparison of these structures reveals that the configuration change of PQQ is induced by the enzymatic reaction. The reaction takes place and the C5-reduced PQQ intermediate is produced when the enzyme co-crystallizes with methanol, but the enzymatic reaction does not take place and the PQQ ring retains a planar configuration of the oxidized orthoquinone form when ethanol instead of methanol is present in the crystallization solution.

PubMed: 21356200
DOI: 10.1016/j.bbrc.2011.02.107

実験手法

X-RAY DIFFRACTION (1.5 Å)