2A9L

SOLUTION NMR STRUCTURE OF A SUBSTRATE FOR THE ARCHAEAL PRE-TRNA SPLICING ENDONUCLEASES: THE BULGE-HELIX-BULGE MOTIF, MINIMIZED AVERAGE STRUCTURE

Summary for 2A9L

Entry DOI	10.2210/pdb2a9l/pdb
Descriptor	ABISS7 RNA (1 entity in total)
Functional Keywords	archaeal bulge-helix-bulge motif, pre-trna splicing, archaea, rna structure, ribonucleic acid, rna
Total number of polymer chains	1
Total formula weight	12317.42
Authors	Diener, J.L.,Moore, P.B. (deposition date: 1998-04-07, release date: 1998-06-17, Last modification date: 2024-05-22)
Primary citation	Diener, J.L.,Moore, P.B. Solution structure of a substrate for the archaeal pre-tRNA splicing endonucleases: the bulge-helix-bulge motif. Mol.Cell, 1:883-894, 1998 Cited by PubMed Abstract: The structure of the bulge-helix-bulge motif that constitutes the intron/exon splice site in H. volcanii pre-tRNATrp has been determined by NMR spectroscopy. The conformations of the two 3 nt bulges, where the pre-tRNA is cleaved, are stabilized by stacking interactions between bulge nucleotides and bases in the adjacent Watson-Crick helices and by a network of backbone hydrogen bonds. Both bulges are presented on the same minor groove face of the central 4 bp helix, and the overall structure has approximate two-fold symmetry, which makes it well-suited for attack by archaeal splicing endonucleases, which are symmetric dimers. PubMed: 9660971 DOI: 10.1016/S1097-2765(00)80087-8 PDB entries with the same primary citation
Experimental method	SOLUTION NMR

Structure validation