2A64

Crystal Structure of Bacterial Ribonuclease P RNA

2A64 の概要

• エントリーDOI: 10.2210/pdb2a64/pdb
• 分子名称: ribonuclease P RNA (1 entity in total)
• 機能のキーワード: hydrolase, ribonuclease, ribozyme, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 135304.17

構造登録者

Kazantsev, A.V.,Krivenko, A.A.,Harrington, D.J.,Holbrook, S.R.,Adams, P.D.,Pace, N.R. (登録日: 2005-07-01, 公開日: 2005-09-20, 最終更新日: 2024-02-14)

主引用文献

Kazantsev, A.V.,Krivenko, A.A.,Harrington, D.J.,Holbrook, S.R.,Adams, P.D.,Pace, N.R.
Crystal structure of a bacterial ribonuclease P RNA.
Proc.Natl.Acad.Sci.Usa, 102:13392-13397, 2005

PubMed Abstract: The x-ray crystal structure of a 417-nt ribonuclease P RNA from Bacillus stearothermophilus was solved to 3.3-A resolution. This RNA enzyme is constructed from a number of coaxially stacked helical domains joined together by local and long-range interactions. These helical domains are arranged to form a remarkably flat surface, which is implicated by a wealth of biochemical data in the binding and cleavage of the precursors of transfer RNA substrate. Previous photoaffinity crosslinking data are used to position the substrate on the crystal structure and to identify the chemically active site of the ribozyme. This site is located in a highly conserved core structure formed by intricately interlaced long-range interactions between interhelical sequences.

PubMed: 16157868
DOI: 10.1073/pnas.0506662102

実験手法

X-RAY DIFFRACTION (3.3 Å)