2ZMV

Crystal structure of Synbindin

Summary for 2ZMV

• Entry DOI: 10.2210/pdb2zmv/pdb
• Descriptor: Trafficking protein particle complex subunit 4 (1 entity in total)
• Functional Keywords: longin domain, synbindin, endoplasmic reticulum, er-golgi transport, golgi apparatus, transport, transport protein
• Biological source: Homo sapiens (Human)
• Cellular location: Golgi apparatus, cis-Golgi network : Q9Y296
• Total number of polymer chains: 2
• Total formula weight: 50874.26

Authors

Fan, F. (deposition date: 2008-04-21, release date: 2008-07-01, Last modification date: 2024-03-13)

Primary citation

Fan, S.,Wei, Z.,Xu, H.,Gong, W.
Crystal structure of human synbindin reveals two conformations of longin domain
Biochem.Biophys.Res.Commun., 378:338-343, 2009

PubMed Abstract: Transport protein particle (TRAPP) is a large multiprotein complex that involves in ER-to-Golgi and intra-Golgi traffic. Synbindin, the human ortholog of yeast Trs23, is one component of the TRAPP complexes. In the hippocampal neurons the synbindin/syndecan complex is involved in synaptic membrane trafficking and thereby regulates the formation of dendritic spines. Here we present the three-dimensional structure of human synbindin, which contains a longin domain (LD) and an atypical PDZ domain (APD). In the crystal, synbindin forms a hexamer, in which the LD forms two different conformations and the APD is quite disordered. These conformational changes of synbindin suggest a possible interaction mode of the LD.

PubMed: 18466758
DOI: 10.1016/j.bbrc.2008.04.143

Experimental method

X-RAY DIFFRACTION (2.8 Å)