2ZMV

Crystal structure of Synbindin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-19
Detector	MAR CCD 165 mm
Wavelength(s)	0.9787, 0.9500, 0.9793
Spacegroup name	P 21 3
Unit cell lengths	124.391, 124.391, 124.391
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.170 - 2.800
R-factor	0.24512
Rwork	0.243
R-free	0.28962
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.067
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Rmerge	0.044
Number of reflections	16132
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	2% MPD, 3% EG, 1% PEG 8000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	2% MPD, 3% EG, 1% PEG 8000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	2% MPD, 3% EG, 1% PEG 8000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K