2YT4
Crystal structure of human DGCR8 core
Summary for 2YT4
| Entry DOI | 10.2210/pdb2yt4/pdb |
| Descriptor | Protein DGCR8 (2 entities in total) |
| Functional Keywords | dsrbd, rna binding domain, rna binding protein |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 26247.09 |
| Authors | Cho, Y.,Sohn, S.Y. (deposition date: 2007-04-05, release date: 2007-08-21, Last modification date: 2024-03-13) |
| Primary citation | Sohn, S.Y.,Bae, W.J.,Kim, J.J.,Yeom, K.H.,Kim, V.N.,Cho, Y. Crystal structure of human DGCR8 core Nat.Struct.Mol.Biol., 14:847-853, 2007 Cited by PubMed Abstract: A complex of Drosha with DGCR8 (or its homolog Pasha) cleaves primary microRNA (pri-miRNA) substrates into precursor miRNA and initiates the microRNA maturation process. Drosha provides the catalytic site for this cleavage, whereas DGCR8 or Pasha provides a frame for anchoring substrate pri-miRNAs. To clarify the molecular basis underlying recognition of pri-miRNA by DGCR8 and Pasha, we determined the crystal structure of the human DGCR8 core (DGCR8S, residues 493-720). In the structure, the two double-stranded RNA-binding domains (dsRBDs) are arranged with pseudo two-fold symmetry and are tightly packed against the C-terminal helix. The H2 helix in each dsRBD is important for recognition of pri-miRNA substrates. This structure, together with fluorescent resonance energy transfer and mutational analyses, suggests that the DGCR8 core recognizes pri-miRNA in two possible orientations. We propose a model for DGCR8's recognition of pri-miRNA. PubMed: 17704815DOI: 10.1038/nsmb1294 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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