4V5D

Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A- and P-site tRNAs, and E-site tRNA.

これはPDB形式変換不可エントリーです。

4V5D の概要

• エントリーDOI: 10.2210/pdb4v5d/pdb
• 関連するPDBエントリー: 1DV4 1EG0 1EMI 1FJG 1FKA 1G1X 1GIX 1HNW 1HNX 1HNZ 1HR0 1I94 1I95 1I96 1I97 1IBK 1IBL 1IBM 1J5E 1JGO 1JGP 1JGQ 1L1U 1N32 1N33 1N34 1N36 1PN7 1PN8 1PNS 1PNX 1QD7 1QZC 1RSS 1TWT 1VOV 1XMO 1XMQ 1XNQ 1XNR 1YL4 2B64 2B9M 2B9O 2F4V 2J00 2J02 2JL5 2JL7 2UU9 2UUA 2UUB 2UUC 2UXB 2UXC 2UXD 2V46 2V48 2VQE 2VQF 2WDG 2WDH 2WDI 2WDJ
• 分子名称: 16S ribosomal RNA, 30S RIBOSOMAL PROTEIN S10, 30S RIBOSOMAL PROTEIN S11, ... (59 entities in total)
• 機能のキーワード: metal-binding, ribosomal protein, ribonucleoprotein, translation, trna-binding, rrna-binding, trna, mrna, ribosome, rna-binding, zinc-finger
• 由来する生物種: THERMUS THERMOPHILUS; 詳細
• タンパク質・核酸の鎖数: 114
• 化学式量合計: 4516213.80

構造登録者

Voorhees, R.M.,Weixlbaumer, A.,Loakes, D.,Kelley, A.C.,Ramakrishnan, V. (登録日: 2009-03-24, 公開日: 2014-07-09, 最終更新日: 2024-11-20)

主引用文献

Voorhees, R.M.,Weixlbaumer, A.,Loakes, D.,Kelley, A.C.,Ramakrishnan, V.
Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome.
Nat. Struct. Mol. Biol., 16:528-533, 2009

PubMed Abstract: Protein synthesis is catalyzed in the peptidyl transferase center (PTC), located in the large (50S) subunit of the ribosome. No high-resolution structure of the intact ribosome has contained a complete active site including both A- and P-site tRNAs. In addition, although past structures of the 50S subunit have found no ordered proteins at the PTC, biochemical evidence suggests that specific proteins are capable of interacting with the 3' ends of tRNA ligands. Here we present structures, at 3.6-A and 3.5-A resolution respectively, of the 70S ribosome in complex with A- and P-site tRNAs that mimic pre- and post-peptidyl-transfer states. These structures demonstrate that the PTC is very similar between the 50S subunit and the intact ribosome. They also reveal interactions between the ribosomal proteins L16 and L27 and the tRNA substrates, helping to elucidate the role of these proteins in peptidyl transfer.

PubMed: 19363482
DOI: 10.1038/nsmb.1577

実験手法

X-RAY DIFFRACTION (3.5 Å)