4V5D
Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A- and P-site tRNAs, and E-site tRNA.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2008-07-20 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 210.864, 450.456, 628.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.500 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2J00 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.500 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.200 | 0.970 |
Number of reflections | 789136 | |
<I/σ(I)> | 7.42 | 1.04 |
Completeness [%] | 97.1 | 75 |
Redundancy | 5.6 | 2.48 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.1 | pH 7.1 |