Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

2K7Z

Solution Structure of the Catalytic Domain of Procaspase-8

Summary for 2K7Z
Entry DOI10.2210/pdb2k7z/pdb
NMR InformationBMRB: 15932
DescriptorCaspase-8 (1 entity in total)
Functional Keywordscaspase, apoptosis, initiator caspase, procaspase, cytoplasm, hydrolase, protease, thiol protease, zymogen
Biological sourceHomo sapiens (Human)
Total number of polymer chains1
Total formula weight30345.42
Authors
Keller, N.,Zerbe, O.,Mares, J.,Gruetter, M.G. (deposition date: 2008-08-28, release date: 2009-03-24, Last modification date: 2024-05-08)
Primary citationKeller, N.,Mares, J.,Zerbe, O.,Grutter, M.G.
Structural and biochemical studies on procaspase-8: new insights on initiator caspase activation.
Structure, 17:438-448, 2009
Cited by
PubMed Abstract: Caspases are proteases with an active-site cysteine and aspartate specificity in their substrates. They are involved in apoptotic cell death and inflammation, and dysfunction of these enzymes is directly linked to a variety of diseases. Caspase-8 initiates an apoptotic pathway triggered by external stimuli. It was previously characterized in its active inhibitor bound state by crystallography. Here we present the solution structure of the monomeric unprocessed catalytic domain of the caspase-8 zymogen, procaspase-8, showing for the first time the position of the linker and flexibility of the active site forming loops. Biophysical studies of carefully designed mutants allowed disentangling dimerization and processing, and we could demonstrate lack of activity of monomeric uncleaved procaspase-8 and of a processed but dimerization-incompetent mutant. The data provide experimental support in so-far unprecedented detail, and reveal why caspase-8 (and most likely other initiator caspases) needs the dimerization platform during activation.
PubMed: 19278658
DOI: 10.1016/j.str.2008.12.019
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

227561

PDB entries from 2024-11-20

PDB statisticsPDBj update infoContact PDBjnumon