2ISP

Ternary complex of DNA Polymerase beta with a dideoxy terminated primer and 2'-deoxyguanosine 5'-beta, gamma-methylene triphosphate

Summary for 2ISP

• Entry DOI: 10.2210/pdb2isp/pdb
• Related: 2ISO
• Descriptor: 5'-D(*CP*CP*GP*AP*CP*CP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3', 5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*(DOC))-3', 5'-D(P*GP*TP*CP*GP*G)-3', ... (9 entities in total)
• Functional Keywords: nucleotidyl transferase, dna polymerase, leaving-group, transferase-dna complex, transferase/dna
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 4
• Total formula weight: 48427.59

Authors

Sucato, C.A.,Upton, T.G.,Kashemirov, B.A.,Martinek, V.,Xiang, Y.,Beard, W.A. (deposition date: 2006-10-18, release date: 2007-01-30, Last modification date: 2023-08-30)

• Primary citation: Sucato, C.A.,Upton, T.G.,Kashemirov, B.A.,Batra, V.K.,Martinek, V.,Xiang, Y.,Beard, W.A.,Pedersen, L.C.,Wilson, S.H.,McKenna, C.E.,Florian, J.,Warshel, A.,Goodman, M.F.; Modifying the beta,gamma Leaving-Group Bridging Oxygen Alters Nucleotide Incorporation Efficiency, Fidelity, and the Catalytic Mechanism of DNA Polymerase beta.; Biochemistry, 46:461-471, 2007; PubMed: 17209556; DOI: 10.1021/bi061517b

Experimental method

X-RAY DIFFRACTION (2.2 Å)