4V50

Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA.

This is a non-PDB format compatible entry.

Summary for 4V50

• Entry DOI: 10.2210/pdb4v50/pdb
• Related: 2I2T 2I2U 2I2V
• Descriptor: 16S ribosomal RNA, 30S ribosomal protein S8, 30S ribosomal protein S9, ... (57 entities in total)
• Functional Keywords: 30s ribosomal subunit, protein-nucleic acid complexes, ribosome
• Biological source: Escherichia coli; More
• Total number of polymer chains: 108
• Total formula weight: 4309661.23

Authors

Berk, V.,Zhang, W.,Pai, R.D.,Cate, J.H.D. (deposition date: 2006-08-16, release date: 2014-07-09, Last modification date: 2023-09-20)

Primary citation

Berk, V.,Zhang, W.,Pai, R.D.,Cate, J.H.D.
Structural basis for mRNA and tRNA positioning on the ribosome.
Proc.Natl.Acad.Sci.Usa, 103:15830-15834, 2006

PubMed Abstract: Protein synthesis requires the accurate positioning of mRNA and tRNA in the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal structures of the intact bacterial ribosome from Escherichia coli in a complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-A resolution, these structures reveal rearrangements in the intact ribosome that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.

PubMed: 17038497
DOI: 10.1073/pnas.0607541103

Experimental method

X-RAY DIFFRACTION (3.22 Å)