4V50
Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 211.783, 395.223, 744.383 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.000 - 3.220 |
| Rwork | 0.287 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 2AVY 2AW4 2AW7 2AWB |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.937 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 3.260 |
| High resolution limit [Å] | 3.220 | 3.220 |
| Number of reflections | 753057 | |
| Completeness [%] | 75.5 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 7.5 | 277 | MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS, EDTA, pH 7.5, batch, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | MPD | ||
| 11 | 1 | 2 | PEG 8000 | ||
| 12 | 1 | 2 | MgCl2 | ||
| 13 | 1 | 2 | NH4Cl | ||
| 2 | 1 | 1 | PEG 8000 | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | NH4Cl | ||
| 5 | 1 | 1 | spermine | ||
| 6 | 1 | 1 | spermidine | ||
| 7 | 1 | 1 | TRIS | ||
| 8 | 1 | 1 | EDTA | ||
| 9 | 1 | 1 | H2O |
