28TI

TraI relaxase in its helicase state in complex with fork DNA (TraI-HEL)

Summary for 28TI

• Entry DOI: 10.2210/pdb28ti/pdb
• EMDB information: 56432 56436
• Descriptor: Multifunctional conjugation protein TraI, DNA (32-MER), DNA (5'-D(P*GP*GP*GP*TP*GP*TP*GP*GP*TP*GP*TP*TP*TP*TP*TP*GP*GP*TP*GP*GP*TP*GP*AP*G)-3') (3 entities in total)
• Functional Keywords: trai; bacterial conjugation; cryo-electron microscopy; relaxase; structural biology; type iv secretion system, transferase
• Biological source: Escherichia coli K-12; More
• Total number of polymer chains: 3
• Total formula weight: 222644.33

Authors

Gorenkin, D.,Liang, R.,Patkowski, J.B.,Bodet-Lefevre, S.,Costa, T.R.D.,Ilangovan, A. (deposition date: 2026-02-18, release date: 2026-11-11)

• Primary citation: Gorenkin, D.,Liang, R.,Patkowski, J.B.,Bodet-Lefevre, S.,Costa, T.R.D.,Ilangovan, A.; Relaxase asymmetry drives initiation of bacterial conjugation; Nat Commun, 2026; DOI: 10.1038/s41467-026-78888-w

Experimental method

ELECTRON MICROSCOPY (3.5 Å)