272D

PARALLEL AND ANTIPARALLEL (G.GC)2 TRIPLE HELIX FRAGMENTS IN A CRYSTAL STRUCTURE

272D の概要

• エントリーDOI: 10.2210/pdb272d/pdb
• 分子名称: DNA (5'-D(*GP*GP*CP*CP*AP*AP*TP*TP*GP*G)-3') (2 entities in total)
• 機能のキーワード: b-dna, double helix, intermolecular base triplet, overhanging bases, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6170.06

構造登録者

Vlieghe, D.,Van Meervelt, L.,Dautant, A.,Gallois, B.,Precigoux, G.,Kennard, O. (登録日: 1996-07-09, 公開日: 1996-09-20, 最終更新日: 2024-02-14)

主引用文献

Vlieghe, D.,Van Meervelt, L.,Dautant, A.,Gallois, B.,Precigoux, G.,Kennard, O.
Parallel and antiparallel (G.GC)2 triple helix fragments in a crystal structure.
Science, 273:1702-1705, 1996

PubMed Abstract: Nucleic acid triplexes are formed by sequence-specific interactions between single-stranded polynucleotides and the double helix. These triplexes are implicated in genetic recombination in vivo and have application to areas that include genome analysis and antigene therapy. Despite the importance of the triple helix, only limited high-resolution structural information is available. The x-ray crystal structure of the oligonucleotide d(GGCCAATTGG) is described; it was designed to contain the d(G middle dotGC)2 fragment and thus provide the basic repeat unit of a DNA triple helix. Parameters derived from this crystal structure have made it possible to construct models of both parallel and antiparallel triple helices.

PubMed: 8781231

実験手法

X-RAY DIFFRACTION (2 Å)