24PI

Ancestrally reconstructed acetolactate synthase - Ancestor N1774 with bound ThDP and magnesium.

Summary for 24PI

• Entry DOI: 10.2210/pdb24pi/pdb
• Descriptor: Ancestral acetolactate synthase, catabolic, MAGNESIUM ION, THIAMINE DIPHOSPHATE, ... (6 entities in total)
• Functional Keywords: thdp, ancestor, transferase
• Biological source: synthetic construct
• Total number of polymer chains: 4
• Total formula weight: 247221.81

Authors

Douw, A.,Guddat, L.W. (deposition date: 2026-03-15, release date: 2026-03-25)

• Primary citation: Douw, A.,Krco, S.,Bayaraa, T.,Foley, G.,Davis, S.J.,Paredes Trujillo, O.,Asser, L.,Sieber, V.,Boden, M.,Hine, D.,Guddat, L.W.,Schenk, G.; Engineering of Acetolactate Synthase for Isobutanol Production Using Ancestral Sequence Reconstruction; To Be Published,

Experimental method

X-RAY DIFFRACTION (2.75 Å)