247D

CRYSTAL STRUCTURES OF AN A-FORM DUPLEX WITH SINGLE-ADENOSINE BULGES AND A CONFORMATIONAL BASIS FOR SITE SPECIFIC RNA SELF-CLEAVAGE

Summary for 247D

• Entry DOI: 10.2210/pdb247d/pdb
• Descriptor: DNA/RNA (5'-R(*GP*CP*GP*)-D(*AP*TP*AP*TP*AP*)-R(*CP*GP*C)-3') (2 entities in total)
• Functional Keywords: a-dna/rna, double helix, bulges, dna-rna complex, dna/rna
• Total number of polymer chains: 1
• Total formula weight: 3454.21

Authors

Portmann, S.,Grimm, S.,Workman, C.,Usman, N.,Egli, M. (deposition date: 1996-02-02, release date: 1996-03-08, Last modification date: 2024-02-14)

Primary citation

Portmann, S.,Grimm, S.,Workman, C.,Usman, N.,Egli, M.
Crystal structures of an A-form duplex with single-adenosine bulges and a conformational basis for site-specific RNA self-cleavage.
Chem.Biol., 3:173-184, 1996

PubMed Abstract: Bulged nucleotides are common secondary structural motifs in RNA molecules and are often involved in RNA-RNA and RNA-protein interactions. RNA is selectively cleaved at bulge sites (when compared to other sites within stems) in the presence of divalent metal cations. The effects of bulge nucleotides on duplex stability and topology have been extensively investigated, but no detailed X-ray structures of bulge-containing RNA fragments have been available.

PubMed: 8807843
DOI: 10.1016/S1074-5521(96)90260-4

Experimental method

X-RAY DIFFRACTION (2.8 Å)