244D

THE HIGH-RESOLUTION CRYSTAL STRUCTURE OF A PARALLEL-STRANDED GUANINE TETRAPLEX

244D の概要

• エントリーDOI: 10.2210/pdb244d/pdb
• 分子名称: DNA (5'-D(*TP*GP*GP*GP*GP*T)-3'), SODIUM ION, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: unusual dna, quadruple helix, parallel-stranded tetraplex, dna
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 30766.58

構造登録者

Laughlan, G.,Murchie, A.I.H.,Norman, D.G.,Moore, M.H.,Moody, P.C.E.,Lilley, D.M.J.,Luisi, B. (登録日: 1995-10-19, 公開日: 1996-02-23, 最終更新日: 2024-02-14)

主引用文献

Laughlan, G.,Murchie, A.I.,Norman, D.G.,Moore, M.H.,Moody, P.C.,Lilley, D.M.,Luisi, B.
The high-resolution crystal structure of a parallel-stranded guanine tetraplex.
Science, 265:520-524, 1994

PubMed Abstract: Repeat tracts of guanine bases found in DNA and RNA can form tetraplex structures in the presence of a variety of monovalent cations. Evidence suggests that guanine tetraplexes assume important functions within chromosomal telomeres, immunoglobulin switch regions, and the human immunodeficiency virus genome. The structure of a parallel-stranded tetraplex formed by the hexanucleotide d(TG4T) and stabilized by sodium cations was determined by x-ray crystallography to 1.2 angstroms resolution. Sharply resolved sodium cations were found between and within planes of hydrogen-bonded guanine quartets, and an ordered groove hydration was observed. Distinct intra- and intermolecular stacking arrangements were adopted by the guanine quartets. Thymine bases were exclusively involved in making extensive lattice contacts.

PubMed: 8036494

実験手法

X-RAY DIFFRACTION (1.2 Å)