23VV

Cryo-EM structure of the UnCas12m4a-crRNA-tgDNA ternary complex in the NTS-cleaving state

Summary for 23VV

• Entry DOI: 10.2210/pdb23vv/pdb
• EMDB information: 69298
• Descriptor: DNA (39-MER), UnCas12m4a, RNA (56-MER), ... (7 entities in total)
• Functional Keywords: nuclease, dna binding protein
• Biological source: bacterium; More
• Total number of polymer chains: 4
• Total formula weight: 115760.34

Authors

Morinaga, H.,Omura, S.N.,Nureki, O. (deposition date: 2026-02-21, release date: 2026-09-02)

• Primary citation: Morinaga, H.,Omura, S.N.,Alfonse, L.,Ornstein, A.,Kobayashi, T.A.,Onishi, K.,Makarova, K.S.,Shmakov, S.A.,Munoz, G.,Garrity, A.J.,DiTommaso, T.,Koonin, E.V.,Maben, Z.,Nureki, O.; Intact RuvC catalytic site and bipartite ssDNA kinking are required for Cas12m nuclease activation; To Be Published,

Experimental method

ELECTRON MICROSCOPY (2.84 Å)