22ID

Structure of Chaetomium thermophilum Ufd1 UT3 domain fused with a ubiquitin C-terminal peptide

Summary for 22ID

• Entry DOI: 10.2210/pdb22id/pdb
• Descriptor: Putative ubiquitin fusion degradation protein (2 entities in total)
• Functional Keywords: ubiquitin, ufd1, protein unfolding, protein binding
• Biological source: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)
• Total number of polymer chains: 2
• Total formula weight: 46058.86

Authors

Wang, Y.,Ji, Z. (deposition date: 2026-01-12, release date: 2026-09-23)

Primary citation

Wang, Y.,Zhang, Z.,He, W.,Wang, P.,Du, J.,Pan, J.,Feng, S.,Huang, J.,Ji, Z.
ATP-independent unfolding of ubiquitin by Ufd1 initiates Cdc48/p97-mediated substrate processing.
Nat.Struct.Mol.Biol., 2026

PubMed Abstract: The Cdc48 ATPase (p97 or VCP in mammals) cooperates with its cofactors Ufd1 and Npl4 to extract polyubiquitinated proteins from membranes or multisubunit complexes, promoting their proteasomal degradation. A ubiquitin molecule in the chain is unfolded in an ATP-independent manner and initiates substrate translocation through the central pore of the ATPase. How ubiquitin is unfolded remains unclear. Here we demonstrate that the UT3 domain of Ufd1 specifically interacts with two K48-linked ubiquitins and unfolds one of the ubiquitins by binding its C-terminal β-strand into a conserved hydrophobic cleft. Simultaneous binding of UT3 to two ubiquitin molecules is required to overcome the energy barrier of ubiquitin unfolding. Subsequently, the UT3-unfolded ubiquitin is captured by Npl4 and Cdc48/p97. Experiments in vitro and in cells show that unfolding-defective mutants of Ufd1 indeed compromise Cdc48/p97 function. Our results provide a reasonable explanation of how simple protein-protein interactions cause the unfolding of the remarkably stable ubiquitin.

PubMed: 42722750
DOI: 10.1038/s41594-026-01884-7

Experimental method

X-RAY DIFFRACTION (2.14 Å)