1ZV8

A structure-based mechanism of SARS virus membrane fusion

1ZV8 の概要

• エントリーDOI: 10.2210/pdb1zv8/pdb
• 関連するPDBエントリー: 1ZV7 1ZVA 1ZVB
• 分子名称: E2 glycoprotein, SODIUM ION, CACODYLATE ION, ... (7 entities in total)
• 機能のキーワード: sars coronavirus, membrane fusion, s2, virus entry, coiled coils, conformational change, viral protein
• 由来する生物種: SARS coronavirus; 詳細
• 細胞内の位置: Virion membrane; Single-pass type I membrane protein: P59594 P59594
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 57131.89

構造登録者

Deng, Y.,Liu, J.,Zheng, Q.,Yong, W.,Dai, J.,Lu, M. (登録日: 2005-06-01, 公開日: 2006-05-16, 最終更新日: 2024-02-14)

主引用文献

Deng, Y.,Liu, J.,Zheng, Q.,Yong, W.,Lu, M.
Structures and Polymorphic Interactions of Two Heptad-Repeat Regions of the SARS Virus S2 Protein.
Structure, 14:889-899, 2006

PubMed Abstract: Entry of SARS coronavirus into its target cell requires large-scale structural transitions in the viral spike (S) glycoprotein in order to induce fusion of the virus and cell membranes. Here we describe the identification and crystal structures of four distinct alpha-helical domains derived from the highly conserved heptad-repeat (HR) regions of the S2 fusion subunit. The four domains are an antiparallel four-stranded coiled coil, a parallel trimeric coiled coil, a four-helix bundle, and a six-helix bundle that is likely the final fusogenic form of the protein. When considered together, the structural and thermodynamic features of the four domains suggest a possible mechanism whereby the HR regions, initially sequestered in the native S glycoprotein spike, are released and refold sequentially to promote membrane fusion. Our results provide a structural framework for understanding the control of membrane fusion and should guide efforts to intervene in the SARS coronavirus entry process.

PubMed: 16698550
DOI: 10.1016/j.str.2006.03.007

実験手法

X-RAY DIFFRACTION (1.94 Å)