1ZIH

GCAA RNA TETRALOOP, NMR, 10 STRUCTURES

Summary for 1ZIH

• Entry DOI: 10.2210/pdb1zih/pdb
• Related: 1ZIF 1ZIG
• Descriptor: RNA (5'-R(*GP*GP*GP*CP*GP*CP*AP*AP*GP*CP*CP*U)-3') (1 entity in total)
• Functional Keywords: ribonucleic acid, rna, tetraloops, gnra, hairpin
• Total number of polymer chains: 1
• Total formula weight: 3866.38

Authors

Jucker, F.M.,Heus, H.A.,Yip, P.F.,Moors, E.,Pardi, A. (deposition date: 1996-07-27, release date: 1997-03-12, Last modification date: 2024-05-22)

Primary citation

Jucker, F.M.,Heus, H.A.,Yip, P.F.,Moors, E.H.,Pardi, A.
A network of heterogeneous hydrogen bonds in GNRA tetraloops.
J.Mol.Biol., 264:968-980, 1996

PubMed Abstract: RNA hairpin loops containing a GNRA consensus sequence are the most frequently occurring hairpins in a variety of prokaryotic and eukaryotic RNAs. These tetraloops play important functional roles in RNA folding, in RNA-RNA tertiary interactions and as protein binding sites. Homo and heteronuclear NMR spectroscopy have been used to determine the structures of the most abundant members of the GNRA tetraloop family: the GAGA, GCAA and GAAA loops closed by a C-G base pair. Analysis of the structures of these three hairpin loops reveals a network of heterogeneous hydrogen bonds. The loops contain a G-A base pair, a G base-phosphate hydrogen bond and several 2' OH-base hydrogen bonds. These intramolecular interactions and the extensive base stacking in the loop help explain the high thermodynamic stability and give insight into the diverse biological roles of the GNRA RNA hairpins.

PubMed: 9000624
DOI: 10.1006/jmbi.1996.0690

Experimental method

SOLUTION NMR