1ZFU
Plectasin:A peptide antibiotic with therapeutic potential from a saprophytic fungus
Summary for 1ZFU
| Entry DOI | 10.2210/pdb1zfu/pdb |
| Descriptor | Plectasin (1 entity in total) |
| Functional Keywords | defensin, antimicrobial peptide, cysteine stabilized alpha-beta motif, antimicrobial protein |
| Biological source | Pseudoplectania nigrella |
| Cellular location | Secreted (Probable): Q53I06 |
| Total number of polymer chains | 1 |
| Total formula weight | 4415.02 |
| Authors | Mygind, P.H.,Fischer, R.L.,Schnorr, K.,Hansen, M.T.,Sonksen, C.P.,Ludvigsen, S.,Raventos, D.,Buskov, S.,Christensen, B.,De Maria, L.,Taboureau, O.,Yaver, D.,Elvig-Jorgensen, S.G.,Sorensen, M.V.,Christensen, B.E.,Kjaerulf, S.,Frimodt-Moller, N.,Lehrer, R.I.,Zasloff, M.,Kristensen, H.H. (deposition date: 2005-04-20, release date: 2005-10-18, Last modification date: 2024-10-30) |
| Primary citation | Mygind, P.H.,Fischer, R.L.,Schnorr, K.M.,Hansen, M.T.,Sonksen, C.P.,Ludvigsen, S.,Raventos, D.,Buskov, S.,Christensen, B.,De Maria, L.,Taboureau, O.,Yaver, D.,Elvig-Jorgensen, S.G.,Sorensen, M.V.,Christensen, B.E.,Kjaerulff, S.,Frimodt-Moller, N.,Lehrer, R.I.,Zasloff, M.,Kristensen, H.H. Plectasin is a peptide antibiotic with therapeutic potential from a saprophytic fungus. Nature, 437:975-980, 2005 Cited by PubMed Abstract: Animals and higher plants express endogenous peptide antibiotics called defensins. These small cysteine-rich peptides are active against bacteria, fungi and viruses. Here we describe plectasin-the first defensin to be isolated from a fungus, the saprophytic ascomycete Pseudoplectania nigrella. Plectasin has primary, secondary and tertiary structures that closely resemble those of defensins found in spiders, scorpions, dragonflies and mussels. Recombinant plectasin was produced at a very high, and commercially viable, yield and purity. In vitro, the recombinant peptide was especially active against Streptococcus pneumoniae, including strains resistant to conventional antibiotics. Plectasin showed extremely low toxicity in mice, and cured them of experimental peritonitis and pneumonia caused by S. pneumoniae as efficaciously as vancomycin and penicillin. These findings identify fungi as a novel source of antimicrobial defensins, and show the therapeutic potential of plectasin. They also suggest that the defensins of insects, molluscs and fungi arose from a common ancestral gene. PubMed: 16222292DOI: 10.1038/nature04051 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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