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1ZC8

Coordinates of tmRNA, SmpB, EF-Tu and h44 fitted into Cryo-EM map of the 70S ribosome and tmRNA complex

Summary for 1ZC8
Entry DOI10.2210/pdb1zc8/pdb
Related1B23 1K8H 1N32
EMDB information1122
DescriptorTLD 16S ribosomal RNA, SsrA-binding protein, Elongation factor Tu, ... (11 entities in total)
Functional Keywordssmpb, tmrna, ef-tu, h44, 30s, 16s rrna, cryo-em, trans-translation, 70s, rna binding protein-rna complex, rna binding protein/rna
Biological sourceThermus thermophilus
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Total number of polymer chains11
Total formula weight194001.90
Authors
Valle, M.,Gillet, R.,Kaur, S.,Henne, A.,Ramakrishnan, V.,Frank, J. (deposition date: 2005-04-11, release date: 2005-04-19, Last modification date: 2024-02-14)
Primary citationValle, M.,Gillet, R.,Kaur, S.,Henne, A.,Ramakrishnan, V.,Frank, J.
Visualizing tmRNA Entry into a Stalled Ribosome
Science, 300:127-130, 2003
Cited by
PubMed Abstract: Bacterial ribosomes stalled on defective messenger RNAs (mRNAs) are rescued by tmRNA, an approximately 300-nucleotide-long molecule that functions as both transfer RNA (tRNA) and mRNA. Translation then switches from the defective message to a short open reading frame on tmRNA that tags the defective nascent peptide chain for degradation. However, the mechanism by which tmRNA can enter and move through the ribosome is unknown. We present a cryo-electron microscopy study at approximately 13 to 15 angstroms of the entry of tmRNA into the ribosome. The structure reveals how tmRNA could move through the ribosome despite its complicated topology and also suggests roles for proteins S1 and SmpB in the function of tmRNA.
PubMed: 12677067
DOI: 10.1126/science.1081798
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (13 Å)
Structure validation

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数据于2025-06-25公开中

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