1ZC8
Coordinates of tmRNA, SmpB, EF-Tu and h44 fitted into Cryo-EM map of the 70S ribosome and tmRNA complex
Summary for 1ZC8
Entry DOI | 10.2210/pdb1zc8/pdb |
Related | 1B23 1K8H 1N32 |
EMDB information | 1122 |
Descriptor | TLD 16S ribosomal RNA, SsrA-binding protein, Elongation factor Tu, ... (11 entities in total) |
Functional Keywords | smpb, tmrna, ef-tu, h44, 30s, 16s rrna, cryo-em, trans-translation, 70s, rna binding protein-rna complex, rna binding protein/rna |
Biological source | Thermus thermophilus More |
Total number of polymer chains | 11 |
Total formula weight | 194001.90 |
Authors | Valle, M.,Gillet, R.,Kaur, S.,Henne, A.,Ramakrishnan, V.,Frank, J. (deposition date: 2005-04-11, release date: 2005-04-19, Last modification date: 2024-02-14) |
Primary citation | Valle, M.,Gillet, R.,Kaur, S.,Henne, A.,Ramakrishnan, V.,Frank, J. Visualizing tmRNA Entry into a Stalled Ribosome Science, 300:127-130, 2003 Cited by PubMed Abstract: Bacterial ribosomes stalled on defective messenger RNAs (mRNAs) are rescued by tmRNA, an approximately 300-nucleotide-long molecule that functions as both transfer RNA (tRNA) and mRNA. Translation then switches from the defective message to a short open reading frame on tmRNA that tags the defective nascent peptide chain for degradation. However, the mechanism by which tmRNA can enter and move through the ribosome is unknown. We present a cryo-electron microscopy study at approximately 13 to 15 angstroms of the entry of tmRNA into the ribosome. The structure reveals how tmRNA could move through the ribosome despite its complicated topology and also suggests roles for proteins S1 and SmpB in the function of tmRNA. PubMed: 12677067DOI: 10.1126/science.1081798 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (13 Å) |
Structure validation
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