1ZC5
Structure of the RNA signal essential for translational frameshifting in HIV-1
Summary for 1ZC5
| Entry DOI | 10.2210/pdb1zc5/pdb |
| NMR Information | BMRB: 6633 |
| Descriptor | HIV-1 frameshift RNA signal (1 entity in total) |
| Functional Keywords | rna bulged helix, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 13250.93 |
| Authors | Gaudin, C.,Mazauric, M.H.,Traikia, M.,Guittet, E.,Yoshizawa, S.,Fourmy, D. (deposition date: 2005-04-11, release date: 2005-06-07, Last modification date: 2024-05-29) |
| Primary citation | Gaudin, C.,Mazauric, M.H.,Traikia, M.,Guittet, E.,Yoshizawa, S.,Fourmy, D. Structure of the RNA Signal Essential for Translational Frameshifting in HIV-1 J.Mol.Biol., 349:1024-1035, 2005 Cited by PubMed Abstract: Many pathogenic viruses use a programmed -1 translational frameshifting mechanism to regulate synthesis of their structural and enzymatic proteins. Frameshifting is vital for viral replication. A slippery sequence bound at the ribosomal A and P sites as well as a downstream stimulatory RNA structure are essential for frameshifting. Conflicting data have been reported concerning the structure of the downstream RNA signal in human immunodeficiency virus type 1 (HIV-1). Here, the solution structure of the HIV-1 frameshifting RNA signal was solved by heteronuclear NMR spectroscopy. This structure reveals a long hairpin fold with an internal three-nucleotide bulge. The internal loop introduces a bend between the lower and upper helical regions, a structural feature often seen in frameshifting pseudoknots. The NMR structure correlates with chemical probing data. The upper stem rich in conserved G-C Watson-Crick base-pairs is highly stable, whereas the bulge region and the lower stem are more flexible. PubMed: 15907937DOI: 10.1016/j.jmb.2005.04.045 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
Download full validation report
