1Z58

Crystal structure of a complex of the ribosome large subunit with rapamycin

Summary for 1Z58

Entry DOI	10.2210/pdb1z58/pdb
Related	1nkw 1ond 1p9x
Descriptor	23S RIBOSOMAL RNA, RAPAMYCIN IMMUNOSUPPRESSANT DRUG (2 entities in total)
Functional Keywords	ribosome, tunnel, polyketyde
Biological source	Deinococcus radiodurans
Total number of polymer chains	1
Total formula weight	934319.17
Authors	Amit, M.,Berisio, R.,Baram, D.,Harms, J.,Bashan, A.,Yonath, A. (deposition date: 2005-03-17, release date: 2005-06-28, Last modification date: 2024-02-14)
Primary citation	Amit, M.,Berisio, R.,Baram, D.,Harms, J.,Bashan, A.,Yonath, A. A crevice adjoining the ribosome tunnel: Hints for cotranslational folding. Febs Lett., 579:3207-3213, 2005 Cited by PubMed Abstract: RNA protection experiments and the crystal structure of a complex of the large ribosomal subunit from the eubacterium Deinococcus radiodurans with rapamycin, a polyketide compound resembling macrolides and ketolides, showed that rapamycin binds to a crevice located at the boundaries of the nascent protein exit tunnel, near its entrance. At this location rapamycin cannot occlude the ribosome exit tunnel, consistent with its failure to act as a ribosomal antibiotic drug. In accord with recent biochemical data, this crevice may play a role in facilitating local cotranslational folding of nascent chains, in particular for transmembrane proteins. PubMed: 15943964 DOI: 10.1016/j.febslet.2005.03.023 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.8 Å)

Structure validation