1YVR
Ro autoantigen
Summary for 1YVR
Entry DOI | 10.2210/pdb1yvr/pdb |
Related | 1YVP |
Descriptor | 60-kDa SS-A/Ro ribonucleoprotein (2 entities in total) |
Functional Keywords | heat repeat; von willebrand factor a; rossmann fold; midas motif', rna binding protein |
Biological source | Xenopus laevis (African clawed frog) |
Cellular location | Cytoplasm: P42700 |
Total number of polymer chains | 1 |
Total formula weight | 60772.38 |
Authors | Stein, A.J.,Fuchs, G.,Fu, C.,Wolin, S.L.,Reinisch, K.M. (deposition date: 2005-02-16, release date: 2005-06-07, Last modification date: 2024-02-14) |
Primary citation | Stein, A.J.,Fuchs, G.,Fu, C.,Wolin, S.L.,Reinisch, K.M. Structural Insights into RNA Quality Control: The Ro Autoantigen Binds Misfolded RNAs via Its Central Cavity Cell(Cambridge,Mass.), 121:529-539, 2005 Cited by PubMed Abstract: The Ro 60 kDa autoantigen is a major target of the immune response in patients with systemic lupus erythematosus. In vertebrate cells, Ro binds misfolded small RNAs and likely functions in RNA quality control. In eukaryotes and bacteria, Ro also associates with small RNAs called Y RNAs. We present structures of unliganded Ro and Ro complexed with two RNAs at 1.95 and 2.2 A resolution, respectively. Ro consists of a von Willebrand factor A domain and a doughnut-shaped domain composed of HEAT repeats. In the complex, a fragment of Y RNA binds on the outer surface of the HEAT-repeat ring, and single-stranded RNA binds in the toroid hole. Mutagenesis supports a binding site for misfolded RNAs that encompasses both sites, with a single-stranded end inserted into the toroid cavity. Our experiments suggest that one role of Y RNAs may be to regulate access of other RNAs to Ro. PubMed: 15907467DOI: 10.1016/j.cell.2005.03.009 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.95 Å) |
Structure validation
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