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1YVR

Ro autoantigen

Summary for 1YVR
Entry DOI10.2210/pdb1yvr/pdb
Related1YVP
Descriptor60-kDa SS-A/Ro ribonucleoprotein (2 entities in total)
Functional Keywordsheat repeat; von willebrand factor a; rossmann fold; midas motif', rna binding protein
Biological sourceXenopus laevis (African clawed frog)
Cellular locationCytoplasm: P42700
Total number of polymer chains1
Total formula weight60772.38
Authors
Stein, A.J.,Fuchs, G.,Fu, C.,Wolin, S.L.,Reinisch, K.M. (deposition date: 2005-02-16, release date: 2005-06-07, Last modification date: 2024-02-14)
Primary citationStein, A.J.,Fuchs, G.,Fu, C.,Wolin, S.L.,Reinisch, K.M.
Structural Insights into RNA Quality Control: The Ro Autoantigen Binds Misfolded RNAs via Its Central Cavity
Cell(Cambridge,Mass.), 121:529-539, 2005
Cited by
PubMed Abstract: The Ro 60 kDa autoantigen is a major target of the immune response in patients with systemic lupus erythematosus. In vertebrate cells, Ro binds misfolded small RNAs and likely functions in RNA quality control. In eukaryotes and bacteria, Ro also associates with small RNAs called Y RNAs. We present structures of unliganded Ro and Ro complexed with two RNAs at 1.95 and 2.2 A resolution, respectively. Ro consists of a von Willebrand factor A domain and a doughnut-shaped domain composed of HEAT repeats. In the complex, a fragment of Y RNA binds on the outer surface of the HEAT-repeat ring, and single-stranded RNA binds in the toroid hole. Mutagenesis supports a binding site for misfolded RNAs that encompasses both sites, with a single-stranded end inserted into the toroid cavity. Our experiments suggest that one role of Y RNAs may be to regulate access of other RNAs to Ro.
PubMed: 15907467
DOI: 10.1016/j.cell.2005.03.009
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.95 Å)
Structure validation

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