1YTF

YEAST TFIIA/TBP/DNA COMPLEX

Summary for 1YTF

• Entry DOI: 10.2210/pdb1ytf/pdb
• Descriptor: DNA (5'-D(*TP*GP*TP*AP*TP*GP*TP*AP*TP*AP*TP*AP*AP*AP*AP*C)-3'), DNA (5'-D(*GP*TP*TP*TP*TP*AP*TP*AP*TP*AP*CP*AP*TP*AP*CP*A)-3'), PROTEIN (TATA BINDING PROTEIN (TBP)), ... (6 entities in total)
• Functional Keywords: transcription regulation, dna, complex, transcription-dna complex, transcription/dna
• Biological source: Saccharomyces cerevisiae (baker's yeast); More
• Cellular location: Nucleus: P13393; Cytoplasm: P32773 P32773 P32774
• Total number of polymer chains: 6
• Total formula weight: 58305.47

Authors

Tan, S.,Hunziker, Y.,Sargent, D.F.,Richmond, T.J. (deposition date: 1996-04-05, release date: 1996-06-20, Last modification date: 2024-02-14)

Primary citation

Tan, S.,Hunziker, Y.,Sargent, D.F.,Richmond, T.J.
Crystal structure of a yeast TFIIA/TBP/DNA complex.
Nature, 381:127-134, 1996

PubMed Abstract: The X-ray crystal structure of the transcription factor IIA (TFIIA) in complex with the TATA-box-binding protein (TBP) and TATA-element DNA is presented at 2.5 A resolution. TFIIA is composed of a beta-barrel and a four-helix bundle motif that together have a boot-like appearance. The beta-barrel extends the TBP beta-sheet and bridges over the DNA major groove immediately upstream of the TATA box. The four-helix bundle contributes substantially to the surface of the complex available for interaction with additional transcription factors.

PubMed: 8610010
DOI: 10.1038/381127a0

Experimental method

X-RAY DIFFRACTION (2.5 Å)