1YTB

CRYSTAL STRUCTURE OF A YEAST TBP/TATA-BOX COMPLEX

1YTB の概要

• エントリーDOI: 10.2210/pdb1ytb/pdb
• 分子名称: DNA (29MER), PROTEIN (TATA BINDING PROTEIN (TBP)) (3 entities in total)
• 機能のキーワード: protein-dna complex, transcription-dna complex, transcription/dna
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Nucleus: P13393
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 58217.14

構造登録者

Kim, Y.,Geiger, J.H.,Hahn, S.,Sigler, P.B. (登録日: 1994-09-28, 公開日: 1995-01-26, 最終更新日: 2024-02-14)

主引用文献

Kim, Y.,Geiger, J.H.,Hahn, S.,Sigler, P.B.
Crystal structure of a yeast TBP/TATA-box complex.
Nature, 365:512-520, 1993

PubMed Abstract: The 2.5 A crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primarily hydrophobic interface with the entire under-surface of the TBP saddle. The severe bend and a positive writhe radically alter the trajectory of the flanking B-form DNA.

PubMed: 8413604
DOI: 10.1038/365512a0

実験手法

X-RAY DIFFRACTION (1.8 Å)