1YLB
NMR solution structure of the reduced spinach plastocyanin
Summary for 1YLB
| Entry DOI | 10.2210/pdb1ylb/pdb |
| Descriptor | Plastocyanin, chloroplast, COPPER (I) ION (2 entities in total) |
| Functional Keywords | plastocyanin, copper(+)-containing, electron-transfer, spinach, photosynthesis, blue-copper protein, electron transport |
| Biological source | Spinacia oleracea (spinach) |
| Cellular location | Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side: P00289 |
| Total number of polymer chains | 1 |
| Total formula weight | 10484.10 |
| Authors | Musiani, F.,Dikiy, A.,Semenov, A.Y.,Ciurli, S. (deposition date: 2005-01-19, release date: 2005-04-05, Last modification date: 2024-05-22) |
| Primary citation | Musiani, F.,Dikiy, A.,Semenov, A.Y.,Ciurli, S. Structure of the Intermolecular Complex between Plastocyanin and Cytochrome f from Spinach. J.Biol.Chem., 280:18833-18841, 2005 Cited by PubMed Abstract: In oxygenic photosynthesis, plastocyanin shuttles electrons between the membrane-bound complexes cytochrome b6f and photosystem I. The homologous complex between cytochrome f and plastocyanin, both from spinach, is the object of this study. The solution structure of the reduced spinach plastocyanin was determined using high field NMR spectroscopy, whereas the model structure of oxidized cytochrome f was obtained by homology modeling calculations and molecular dynamics. The model structure of the intermolecular complex was calculated using the program AUTODOCK, taking into account biological information obtained from mutagenesis experiments. The best electron transfer pathway from the heme group of cytochrome f to the copper ion of plastocyanin was calculated using the program HARLEM, obtaining a coupling decay value of 1.8 x 10(-4). Possible mechanisms of interaction and electron transfer between plastocyanin and cytochrome f were discussed considering the possible formation of a supercomplex that associates one cytochrome b6f, one photosystem I, and one plastocyanin. PubMed: 15691836DOI: 10.1074/jbc.M412760200 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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