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1YI5

Crystal structure of the a-cobratoxin-AChBP complex

1YI5 の概要
エントリーDOI10.2210/pdb1yi5/pdb
関連するPDBエントリー1I9B 1UV6 1UW6 1UX2
分子名称Acetylcholine-binding protein, Long neurotoxin 1 (2 entities in total)
機能のキーワードacetylcholine binding protein, snake three-fingered alpha-neurotoxin, toxin
由来する生物種Lymnaea stagnalis (great pond snail)
詳細
細胞内の位置Secreted: P58154 P01391
タンパク質・核酸の鎖数10
化学式量合計158523.09
構造登録者
Bourne, Y.,Talley, T.T.,Hansen, S.B.,Taylor, P.,Marchot, P. (登録日: 2005-01-11, 公開日: 2005-05-17, 最終更新日: 2024-10-30)
主引用文献Bourne, Y.,Talley, T.T.,Hansen, S.B.,Taylor, P.,Marchot, P.
Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and nicotinic receptors
Embo J., 24:1512-1522, 2005
Cited by
PubMed Abstract: The crystal structure of the snake long alpha-neurotoxin, alpha-cobratoxin, bound to the pentameric acetylcholine-binding protein (AChBP) from Lymnaea stagnalis, was solved from good quality density maps despite a 4.2 A overall resolution. The structure unambiguously reveals the positions and orientations of all five three-fingered toxin molecules inserted at the AChBP subunit interfaces and the conformational changes associated with toxin binding. AChBP loops C and F that border the ligand-binding pocket move markedly from their original positions to wrap around the tips of the toxin first and second fingers and part of its C-terminus, while rearrangements also occur in the toxin fingers. At the interface of the complex, major interactions involve aromatic and aliphatic side chains within the AChBP binding pocket and, at the buried tip of the toxin second finger, conserved Phe and Arg residues that partially mimic a bound agonist molecule. Hence this structure, in revealing a distinctive and unpredicted conformation of the toxin-bound AChBP molecule, provides a lead template resembling a resting state conformation of the nicotinic receptor and for understanding selectivity of curaremimetic alpha-neurotoxins for the various receptor species.
PubMed: 15791209
DOI: 10.1038/sj.emboj.7600620
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (4.2 Å)
構造検証レポート
Validation report summary of 1yi5
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-10-30に公開中

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