1YI5
Crystal structure of the a-cobratoxin-AChBP complex
1YI5 の概要
エントリーDOI | 10.2210/pdb1yi5/pdb |
関連するPDBエントリー | 1I9B 1UV6 1UW6 1UX2 |
分子名称 | Acetylcholine-binding protein, Long neurotoxin 1 (2 entities in total) |
機能のキーワード | acetylcholine binding protein, snake three-fingered alpha-neurotoxin, toxin |
由来する生物種 | Lymnaea stagnalis (great pond snail) 詳細 |
細胞内の位置 | Secreted: P58154 P01391 |
タンパク質・核酸の鎖数 | 10 |
化学式量合計 | 158523.09 |
構造登録者 | Bourne, Y.,Talley, T.T.,Hansen, S.B.,Taylor, P.,Marchot, P. (登録日: 2005-01-11, 公開日: 2005-05-17, 最終更新日: 2024-10-30) |
主引用文献 | Bourne, Y.,Talley, T.T.,Hansen, S.B.,Taylor, P.,Marchot, P. Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and nicotinic receptors Embo J., 24:1512-1522, 2005 Cited by PubMed Abstract: The crystal structure of the snake long alpha-neurotoxin, alpha-cobratoxin, bound to the pentameric acetylcholine-binding protein (AChBP) from Lymnaea stagnalis, was solved from good quality density maps despite a 4.2 A overall resolution. The structure unambiguously reveals the positions and orientations of all five three-fingered toxin molecules inserted at the AChBP subunit interfaces and the conformational changes associated with toxin binding. AChBP loops C and F that border the ligand-binding pocket move markedly from their original positions to wrap around the tips of the toxin first and second fingers and part of its C-terminus, while rearrangements also occur in the toxin fingers. At the interface of the complex, major interactions involve aromatic and aliphatic side chains within the AChBP binding pocket and, at the buried tip of the toxin second finger, conserved Phe and Arg residues that partially mimic a bound agonist molecule. Hence this structure, in revealing a distinctive and unpredicted conformation of the toxin-bound AChBP molecule, provides a lead template resembling a resting state conformation of the nicotinic receptor and for understanding selectivity of curaremimetic alpha-neurotoxins for the various receptor species. PubMed: 15791209DOI: 10.1038/sj.emboj.7600620 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (4.2 Å) |
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