1YBK

RHCC cocrystallized with CAPB

1YBK の概要

• エントリーDOI: 10.2210/pdb1ybk/pdb
• 関連するPDBエントリー: 1fe6
• 分子名称: tetrabrachion, SULFATE ION, N-(CARBOXYMETHYL)-N,N-DIMETHYL-3-[(1-OXODODECYL)AMINO]-1-PROPANAMINIUM INNER SALT, ... (4 entities in total)
• 機能のキーワード: right hand coiled coil, protein binding
• 由来する生物種: Staphylothermus marinus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 24225.59

構造登録者

Stetefeld, J. (登録日: 2004-12-21, 公開日: 2005-01-18, 最終更新日: 2024-02-14)

主引用文献

Ozbek, S.,Muller, J.F.,Figgemeier, E.,Stetefeld, J.
Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB).
Acta Crystallogr.,Sect.D, 61:477-480, 2005

PubMed Abstract: Crystals of excellent quality are a prerequisite for high-resolution X-ray data. However, in refinement protocols of crystallization conditions it is often difficult to obtain the right combination of, for example, protein concentration, drop size, temperature and additives. A novel approach for optimizing crystal contacts in a most favourable fashion by performing crystallization setups with the zwitterionic surfactant cocoamidoproylbetaine (CAPB) is introduced. In the presence of this surfactant, highly diffracting crystals were obtained. Here, data from a right-handed coiled coil (RHCC) in complex with CAPB at 1.4 A resolution are presented. The addition of CAPB using otherwise identical crystallization conditions and the same X-ray source caused an improvement in resolution from 2.9 to 1.4 A.

PubMed: 15805603
DOI: 10.1107/S0907444905001204

実験手法

X-RAY DIFFRACTION (1.45 Å)