1XZ0

Crystal structure of CD1a in complex with a synthetic mycobactin lipopeptide

1XZ0 の概要

• エントリーDOI: 10.2210/pdb1xz0/pdb
• 関連するPDBエントリー: 1CD1 1GZP 1GZQ 1ONQ 1UQS
• 分子名称: T-cell surface glycoprotein CD1a, Beta-2-microglobulin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: beta sheet platform, mhc-fold, protein-lipopeptide complex, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 90306.54

構造登録者

Zajonc, D.M.,Crispin, M.D.,Bowden, T.A.,Young, D.C.,Cheng, T.Y.,Hu, J.,Costello, C.E.,Miller, M.J.,Moody, D.B.,Wilson, I.A. (登録日: 2004-11-11, 公開日: 2005-03-01, 最終更新日: 2024-10-30)

主引用文献

Zajonc, D.M.,Crispin, M.D.,Bowden, T.A.,Young, D.C.,Cheng, T.Y.,Hu, J.,Costello, C.E.,Rudd, P.M.,Dwek, R.A.,Miller, M.J.,Brenner, M.B.,Moody, D.B.,Wilson, I.A.
Molecular Mechanism of Lipopeptide Presentation by CD1a.
Immunity, 22:209-219, 2005

PubMed Abstract: CD1a is expressed on Langerhans cells (LCs) and dendritic cells (DCs), where it mediates T cell recognition of glycolipid and lipopeptide antigens that contain either one or two alkyl chains. We demonstrate here that CD1a-restricted T cells can discriminate the peptide component of didehydroxymycobactin lipopeptides. Structure analysis of CD1a cocrystallized with a synthetic mycobactin lipopeptide at 2.8 A resolution further reveals that the single alkyl chain is inserted deep within the A' pocket of the groove, whereas its two peptidic branches protrude along the F' pocket to the outer, alpha-helical surface of CD1a for recognition by the TCR. Remarkably, the cyclized lysine branch of the peptide moiety lies in the shallow F' pocket in a conformation that closely mimics that of the alkyl chain in the CD1a-sulfatide structure. Thus, this structural study illustrates how a single chain lipid can be presented by CD1 and that the peptide moiety of the lipopeptide is recognized by the TCR.

PubMed: 15723809
DOI: 10.1016/j.immuni.2004.12.009

実験手法

X-RAY DIFFRACTION (2.8 Å)