1XQI

Crystal Structure Analysis of an NDP kinase from Pyrobaculum aerophilum

Summary for 1XQI

• Entry DOI: 10.2210/pdb1xqi/pdb
• Related: 1NPK
• Descriptor: Nucleoside diphosphate kinase, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, TRIETHYLENE GLYCOL, ... (4 entities in total)
• Functional Keywords: alpha/beta sandwich, ferredoxin fold, transferase
• Biological source: Pyrobaculum aerophilum
• Cellular location: Cytoplasm (By similarity): Q8ZWY4
• Total number of polymer chains: 3
• Total formula weight: 66906.03

Authors

Pedelacq, J.D.,Waldo, G.S.,Cabantous, S.,Liong, E.C.,Berendzen, J.,Terwilliger, T.C. (deposition date: 2004-10-12, release date: 2005-09-20, Last modification date: 2024-10-30)

Primary citation

Pedelacq, J.D.,Waldo, G.S.,Cabantous, S.,Liong, E.C.,Terwilliger, T.C.
Structural and functional features of an NDP kinase from the hyperthermophile crenarchaeon Pyrobaculum aerophilum
Protein Sci., 14:2562-2573, 2005

PubMed Abstract: Nucleoside diphosphate (NDP) kinases are ubiquitous enzymes that transfer gamma-phosphates from nucleoside triphosphates to nucleoside diphosphates via a ping-pong mechanism. The important role of this large family of enzymes in controlling cellular functions and developmental processes along with their crystallizability has made them good candidates for structural studies. We recently determined the structure of an evolved version of an NDP kinase from Pyrobaculum aerophilum, an extreme thermophile. This NDP kinase has similarity to the 42 other NDP kinases deposited in the Protein Data Bank (PDB) but differs significantly in sequence, structure, and biophysical properties. The P. aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66-100 and 156-165. We show that deletion mutants of the P. aerophilum NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor. A structural analysis of the evolved NDP kinase in conjunction with mutagenesis experiments suggests that the substrate specificity of the P. aerophilum NDP kinase is related to the presence of these two inserts.

PubMed: 16195547
DOI: 10.1110/ps.051664205

Experimental method

X-RAY DIFFRACTION (2.5 Å)